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You are here:Home » Molecular Biology » Growth Factors-ENA78 » ENA78, Recombinant, Human (Epithelial Neutrophil Activating Peptide 78)

ENA78, Recombinant, Human (Epithelial Neutrophil Activating Peptide 78)

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Specifications

Recombinant Human ENA-78 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8020 Dalton. Recombinant Human ENA-78 is purified by proprietary chromatographic techniques.
Catalog #E2275-06
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be, Ala- Ala -Val-Leu-Arg. .
Dimers and AggregatesLess than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityRecombinant Human ENA-78 is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human peripheral blood neutrophils using a concentration of 5-10 ng/ml.
Endotoxin 0.1ng/ug (IEU/ug) of Recombinant Human Epithelial neutrophil-activating protein 78
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a calibrated solution of ENA-78 as a Reference Standard.
SolubilityIt is recommended to reconstitute the lyophilized ENA-78 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized ENA-78 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution ENA-78 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight8.02kD
SourceE. coli
Purity98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration~1mg/ml
FormSupplied as a lyophilized powder without additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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