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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IC FC

Endomucin (endothelial sialomucin; also Endomucin-1/2 and Mucin-14) is an 80-120kD glycoprotein member of the Endomucin family of proteins. It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or anti-adhesive molecule. Mouse Endomucin precursor is 261 amino acids in length. It is type I transmembrane protein that contains a 170 aa extracellular domain (ECD) (aa 21-190) and a 50 aa cytoplasmic region. Three splice variants exist in the ECD. One shows a deletion of aa 91-141, a second shows a one aa substitution for aa 91-129, and a third shows a one aa substitution for aa 129-142. Over aa 21-90, mouse Endomucin shares 60% and 30% aa identity with rat and human Endomucin, respectively.
Catalog #	E2291-34
Applications	Suitable for use in Flow cytometry, Direct ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution	Flow Cytometry: 25ug/mL use 10ul to label 10e6 cells
	Direct ELISA: 0.5-1ug/mL detection limit for rmEndomucin ~ 0.3ng/well.
	Western Blot: 0.1-0.2ug/mL detection limit for rmEndomucin ~ 1ng/lane under non-reducing and reducing conditions.
	Immunocytochemistry: 10ug/mL
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Mouse
Concentration	As reported
Form	Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 200ul 40-50% glycerol, PBS.
Purity	Purified by immunoaffinity chromatography.
Immunogen	E. coli-derived, recombinant mouse Endomucin (aa 21 - 90).
Specificity	Recognizes mouse Endomucin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.