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You are here:Home » Antibodies » Abs to Enzymes, Endonuclease » Anti -Endonuclease G (EndoG)

Anti -Endonuclease G (EndoG)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is speci cally activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1,2). EndoG is a mitochondrion-speci c nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the rst mitochondrial protein identi ed to be involved in apoptosis in C. elegans (2). EndooG also cleaves DNA in vitro (4).
Catalog #E2291-40
Applications Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution Western Blot: 1-2ug/ml; Optimal dilutions to be determined by the researcher.
Positive Control HepG2 and 3T3 cell lysates
Storage and Stability May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenRaised with a synthetic peptide (GGPRGPGELAKYGLP) corresponding to amino acids 55 to 70 of human EndoG (5).
SpecificityRecognizes a 35kD band. Species Crossreactivity: Mouse and rat.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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