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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

Catalog #	E2291-40A
Recommended Secondary Reagents	Sheep anti Rabbit IgG (Aff. Pur.): HRP
Applications	Western Blotting: 1-2ug/ml
	Optimal working dilutions to be determined by researcher.
Western Blotting	Detects a band of approximately 35kD in HepG2 and 3T3 cell lysates.
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS with 0.02% sodium azide.
Purity	Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen	Synthetic peptide GGPRGPGELAKYGLP corresponding to amino acids 55 - 70 of endonuclease G.
Specificity	Recognizes endonuclease G (endoG), a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis endoG i reported to act independently of caspases and may represent a caspase independent apoptotic pathway initiated from the mitochondria. Species Crossreactivity: Human. Expected crossreactivity from sequence similarity mouse, rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.