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Endonuclease III, Recombinant, E. coli, aa1-211, His-tag (nth, b1633, JW1625, DNA-(apurinic or apyrimidinic site) Lyase)
Endonuclease III, Recombinant, E. coli, aa1-211, His-tag (nth, b1633, JW1625,
DNA-(apurinic or apyrimidinic site) Lyase)
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| Nth, also known as Endonuclease III, has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. This enzyme incises damaged DNA at cytosines, thymines and guanines. Nth acts on a damaged strand, 5' from the damaged site. This enzyme is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions. | | | Catalog # | E2291-41C | | Description | Recombinant E. coli NTH (Endonuclease III) protein fused to His-tag at the N-terminus; expressed in E. coli | | AA Sequence | MGSSHHHHHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I | | Molecular Weight | 25.7kD (231aa) confirmed by MALDI-TOF | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C or lower. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 25.7kD | | Source | E. coli | | Purity | 85% (SDS-PAGE) | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in 20mM Tris-HCl buffer, pH8.0, 0.1M sodium chloride, 1mM DTT, 0.1mM PMSF, 40% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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