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Specifications

Hercules Endonuclease is a engineered endonuclease from Serratia marcescens. The endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. The enzyme completely digests nucleic acids to 5'-monophosphate terminated oligonucleotides 2-5 bases in length.
Catalog #	E2291-42
Source	Purified from an E. coli strain expressing a recombinant clone.
Applications	Viscosity reduction in protein extracts.
	Sample preparation for 2D gel electrophoresis.
	Removal of nucleic acid contaminants from recombinant protein preparations.
Unit Definition	One unit of Hercules Endonuclease is defined as the amount of enzyme that causes an A260 change of 1.0 in 30 minutes at 37°C, which corresponds to complete digestion of 37ug of DNA.
Storage & Stability	Stable at 4°C for several weeks, for long-term storage, store it at -20°C after receiving. No obvious decrease of enzyme activity can be found after an incubation at 37°C for a week.
Source	Serratia marcescens
Purity	99% as determined by SDS-PAGE.
Concentration	~200U/ul
Form	Storage Buffer: 10mM Tris-HCl, pH8.0, 2mM MgCl2, 20mM NaCl, 50% glycerol.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.