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You are here:Home » Antibodies » Abs to Enzymes, Isomerase » Anti -Endoplasmic Reticulum Protein 57 (ERp57, GRP58, PDIA3, Protein disulfide isomerase family A, member 3 [Homo sapiens], HGNC:4606, ERp60, ERp61, GRP57, HsT17083, P58, PI-PLC, 58kD microsomal protein, endoplasmic reticulum P58, glucose regulated protein, 58k

Anti -Endoplasmic Reticulum Protein 57 (ERp57, GRP58, PDIA3, Protein disulfide isomerase
family A, member 3 [Homo sapiens], HGNC:4606, ERp60, ERp61, GRP57, HsT17083, P58, PI-PLC,
58kD microsomal protein, endoplasmic reticulum P58, glucose regulated protein, 58k

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IP
ERp57 (also known as Grp58), a member of the thioredoxin superfamily, is an ER protein with disulfide isomerase activity. It has been shown to increase after oncogenic transformation and to be a component of the MHC Class I peptide-loading complex.
Catalog #E2291-75D
The Grpís are induced by glucose or oxygen deprivation and by chemical compounds which perturb protein trafficking and secretion or calcium homeostasis. It has been reported (2) that the microsomal carnitine palmitoyl-transferase (CPT) seemed to be the protein that had been described previously as Grp58 (3), also known as Erp57, Erp61 (4), and HIP-70 (hormone-induced protein-70) (5). It has since been established that Erp61 and Grp58 are the same protein (6). CPT has a nuclear localization signal (7) and has been localized in the nucleus (8). It has been speculated (1) that in the nuclear microenvironment, by acting as a CPT, this protein modulates the levels of acyl-CoA and carnitine, both of which have been implicated in the regulation of gene transcription (9Ė11). It is also known that protein acylation-deacylation is crucial for the proper functioning of certain proteins and that this requires acyl-CoA (12). Reportedly oestrogen and leutenzing-hormone-releaseing hormone induce this 54kD protein ~100 fold in the hippocampus region of the brain (13). The presence in this protein of an apparent oestrogen-binding domain (14) and of two thioredoxin active-site sequences, CGHC, were recognized previously. Proteins with one or more CGHC sequence are classified as members of the thioredoxin superfamily (15). It remains to be seen how the possible interaction of the oestrogen-binding and CGHC domain, together with the CPT activity, link the diverse apparent functions ascribed to this protein.
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:2000-1:5000 dilution of this lot detected ERp57 in RIPA lysates from HeLa cells. HeLa cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with E2291-75D (1:2000 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No5H39
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid, 0.05% sodium azide before the addition of glycerol to 30%.
PurityAscites
ImmunogenFull length human ERp57, produced in E. coli
SpecificityRecognizes human ERp57, Mr ~57kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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