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You are here:Home » Antibodies » Antibodies-Enzymes, Isomerase » Anti -Endoplasmic Reticulum Protein 72 (ERp72, PDIA4, Protein Disulfide Isomerase A4)

Anti -Endoplasmic Reticulum Protein 72 (ERp72, PDIA4, Protein Disulfide Isomerase A4)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (2). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum stress protein 72 (ERp72), constitute the PDI family (3,4). ERp72 contains three thioredoxin homology domains (3) and plays a role in the formation and isomerization of disulfide bonds on secretory proteins (3,4).
Catalog #E2291-77C
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol.
PurityAffinity chromatography.
ImmunogenSynthetic peptide (KLH-coupled) derived from the sequence of human ERp72.
SpecificityDetects endog-enous levels of total ERp72 protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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