Suitable for use in Immunohistochemistry. Not suitable for use in cell suspensions or Western Blot. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Acetone-fixed frozen sections): 1:100 diluted in PBS. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid, 10mM sodium azide.
Purity
Supernatant
Specificity
Recognizes human Endothelium. Stains human blood vessels with the exception of arteries. Species Crossreactivity: Goat, rabbit, porcine, canine. Does not stain rat, mouse or chicken endothelium.
References
1. Jones, R.R., et al., 1986, J. Clin. Path. 39, 742-749. |2. Holden, C.A., et al., 1986, J. Immun. Meth. 91, 45-52. |3. Schlingemann, R.O., et al., 1987, Laboratory Investigation 52, 71.