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You are here:Home » Antibodies » Antibodies-Cell Markers » Anti -Endothelium

Anti -Endothelium


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
Catalog #E2292-02A
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry (FFPE): 1:20-1:100
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone NoEN4
ConcentrationNot Determined
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman Endothelium
SpecificityRecognizes human Endothelium. Stains all human blood vessels including brain microvessels. Both large and small vessels are equally reactive. The EN4 antigen is preserved in endothelial cells in culture unlike the PAL-E antigen which is lost. It stains strongly mouse fibroblasts transfected with the human CD31 gene. On surface-iodinated Jurkat T cells it recognizes the 130kD CD31 antigen. Also binds to guinea-pig and feline endothelium, but not to rabbit, bovine, sheep, canine, rat or mice endothelial cells. No other structures in the skin, heart, kidney, tonsils or spleen are stained with this antibody.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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