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You are here:Home » Antibodies » Abs to Angiogenesis » Anti -Endostatin

Anti -Endostatin

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Endostatin is a 20kD C-terminal fragment of collagen XVIII. The exact molecular mass determined by ESI-MS was found to be 18,494D. Endostatin specifically inhibits endothelial proliferation and potently inhibits angiogenesis and tumor growth. Anti-endostatin monoclonal antibody administered to nude mice transplanted with human hepatocellular carcinoma cells (JHH-1 line) that endogenously produced endostatin promoted tumor angiogenesis by inhibiting endostatin activity in the tumor and subsequently increased tumor mass by preventing cancer cells from undergoing apoptosis. The ability of endostatin to bind Zn2+ is essential for its antiangiogenic activity. The endostatin precursor collagen XVIII is expressed at high levels in human livers, the main source being hepatocytes. The generation of endostatin or endostatin-like collagen XVIII fragments is catalyzed by proteolytic enzymes, including cathepsin L and matrix metalloproteases, that cleave peptide bonds within the protease-sensitive hinge region of the C-terminal domain. The processing of collagen XVIII to endostatin may represent a local control mechanism for the regulation of angiogenesis. Circulating endostatin was detected in patients with various types of cancer as well as in healthy controls. Circulating endostatin concentrations are significantly increased in patients with systemic sclerosis. Interestingly, pericardial fluid levels of endostatin are associated with the presence (40% lower levels) or absence of collaterals in patients with CAD.
Catalog #E2296-01
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: Indirect
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophillized powder from PBS, pH 7.2. No preservatives added. Reconstitute with 100ul of sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant human Endostatin, a 20kD terminal fragment of collagen XVIII.
SpecificityRecognizes human Endostatin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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