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You are here:Home » Antibodies » Abs to Angiogenesis » Anti -Endostatin

Anti -Endostatin

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Recent studies have identified several proteolytic fragments or cryptic domains of proteins that act as inhibitors of angiogenesis. These include fragments of plasminogen such as Angiostatin protein (kringles 1-4) and kringles 1-5, C-terminal proteolytic fragment of Collagen XVIII (Endostatin protein), the NC10 domain of collagen 15 (Restin), the C-terminal hemopexin-like domain of MMP-2 (PEX), the N-terminal fragment of prolactin, and the N-terminally truncated platelet factor. The 20kD fragment of Collagen XVIII known as Endostatin protein inhibits tumor progression and induction of endothelial cell apoptosis. Administration of Endostatin protein to mice bearing certain tumors caused tumor regression without the development of drug resistance. Endostatin protein also inhibited systemic angiogenesis, primary tumor growth, and the development of primary metastatic lesions. It also reduced the levels of antiapoptotic proteins Bcl-2 and Bcl-xL and an increase in apoptosis of endothelial cells. in vitro.
Catalog #E2296-10Y
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionWestern Blot:1-10ug/ml using Chemiluminescence technique. Human and mouse endostatins are ~ 20kD.
ELISA:1:10K-1:100K; using 50-100 ng of control peptide/well
Optimal dilution determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified human endostatin protein
SpecificitySpecies Crossreactivity: Mouse and rat protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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