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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Endostatin, which corresponds to the C-terminal fragment of collagen XVIII, is a potent inhibitor of endothelial cell proliferation, migration and angiogenesis. Recent study shows that endostatin induces tyrosine kinase activity and enhanced apoptosis in FGF-treated endothelial cells. Endostatin is currently being used in Phase I trails as an antitumor agent.
Catalog #	E2900
Applications	Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions	Western Blot: 1:500-1:2000. Detects endostatin in mouse liver cell lysates to which 50ng of recombinant human endostatin was added. Mouse liver cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with E2900. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
	Optimal dilutions to be determined by the researcher.
Recommended secondary antibodies	I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	4i37
Host	Mouse
Source	Mouse
Concentration	Not Determined
Form	Supplied as a liquid in 50mM sodium phosphate, pH 8.0, 0.05% sodium azide, before the addition of glycerol to 30%.
Purity	Puri ed by Thiophilic chromatography.
Immunogen	Full-length recombinant mouse Endostatin. Species Sequence Homology: rat.
Specificity	Recognizes mouse Endostatin, Mr 22kD, an unidenti ed protein is also weakly detected, Mr 54kD.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.