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Endothelial Cell Growth Supplement (ECGS)
Endothelial Cell Growth Supplement (ECGS)
Pricing
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| ECGS is mitogenic for many types of cells (i.e. mammalian, avian and human endothelial cells, smooth muscle cells, keratinocytes, melanocytes and hybridomas) under reduced or serum-free conditions. It often fully substitutes for feeder layers in culture of fastidious cells. Bovine hypothalamic protein extract, is rich in cell growth and attachment factors. | | | Catalog # | E3010 | | Range for Optimal Activity | Use in culture at 10-100ug/ml | | Biological Activity | 15ug/ml supports 4.6-fold growth of fetal bovine heart endothelial cells cultured for 5 days in 50% RPMI/50% DMEM (RD) medium, 2% FBS. Results may vary depending on cell line used. | | Storage and Stability | Lyophilized powder is stable for 12 months at -20°C. Reconstituted product is stable for 3 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. | | Source | Bovine hypothalamus. | | Purity | Filtered through a 0.2um membrane and packaged aseptically. | | Form | Supplied as a lyophilized powder from sterile water containing trace amounts of sodium chloride, streptomycin sulfate. Reconstitute with sterile medium, physiological buffer or water. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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