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You are here:Home » Growth Factors, Cytokines » Growth Factors-Endothelial » Endothelial Cell Growth Supplement (ECGS)

Endothelial Cell Growth Supplement (ECGS)

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Specifications

ECGS is mitogenic for many types of cells (i.e. mammalian, avian and human endothelial cells, smooth muscle cells, keratinocytes, melanocytes and hybridomas) under reduced or serum-free conditions. It often fully substitutes for feeder layers in culture of fastidious cells. Bovine hypothalamic protein extract, is rich in cell growth and attachment factors.
Catalog #E3010
Range for Optimal ActivityUse in culture at 10-100ug/ml
Biological Activity15ug/ml supports 4.6-fold growth of fetal bovine heart endothelial cells cultured for 5 days in 50% RPMI/50% DMEM (RD) medium, 2% FBS. Results may vary depending on cell line used.
Storage and StabilityLyophilized powder is stable for 12 months at -20°C. Reconstituted product is stable for 3 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
SourceBovine hypothalamus.
PurityFiltered through a 0.2um membrane and packaged aseptically.
FormSupplied as a lyophilized powder from sterile water containing trace amounts of sodium chloride, streptomycin sulfate. Reconstitute with sterile medium, physiological buffer or water.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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