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You are here:Home » Antibodies » Abs to Enzymes, Lipase » Anti -Endothelial Lipase (EL)

Anti -Endothelial Lipase (EL)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Endothelial lipase (EL) is a member of the triglyceride lipase gene family. It functions primarily as a phospholipase and has low triglyceride lipase activity. It has been shown to be a major genetic determinant for the concentration, structure, and metabolism of high-density lipoprotein, which protects against atherosclerosis (1,2). It was originally cloned from endothelial cells and was found to be expressed in a distinct and complementary tissue-restricted fashion, with high-level expression in the liver, placenta, lung, ovary, and macrophage(3). The wide spread distribution of this protein suggests that it plays a general role in lipid metabolism. Immunohistochemical studies demonstrate that EL is expressed in infiltrating cells such as macrophages within atheromatous plaques, in addition to endothelial and smooth muscle cells in non-atherosclerotic coronary arteries. Furthermore, EL expression is detected in the neovasculature within atheromatous plaques in atherosclerotic coronary arteries, indicating that EL may have unique functional roles in atherosclerosis.
Catalog #E3010-36
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 3ug/ml
Immunohistochemistry (FFPE): 2ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlWestern Blot: HepG2 cell lysate
Immunohistochemistry: Paraffin-embedded rat kidney sections.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.6mg/ml
FormSupplied as a liquid in TBS, pH 7.4, 0.5mg/ml BSA, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography
ImmunogenSynthetic peptide corresponding to aa19-32, AGSPVPFGPEGRLE of human endothelial lipase.
SpecificityRecognizes human Endothelial Lipase at ~57kD.
Species CrossreactivityMouse, rat, sheep and porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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