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Endothelial Progenitor Cells, Human, Freezing Medium
Endothelial Progenitor Cells, Human, Freezing Medium
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| Catalog # | E3010-60F | | Description | CD133+ human EPC freezing medium was optimized for cryopreservation of our human endothelium progenitor cells (hEPC). | | This product contains fetal bovine serum. Each lot is tested for sterility. | | Procedure for Freezing EPC | 1. Freeze EPC cells when they reach 90% to 95% confluence. | | 2. Warm Dulbecco’s PBS (without Ca2+ and Mg2+) and 0.05% trypspin/EDTA in a 37°C water bath. Thaw EPOC freezing medium at room temperature. | | 3. Remove EPC growth medium, rinse EPC with Dulbecco’s PBS, and incubate the cells with trypsin/EDTA solution until approximately 90% of the cells begin to detach. Monitor the cells under a microscope to avoid over-trypsinization. | | 4. Add fetal bovine serum equal to 1/10 volume of the trypsin/EDTA solution to neutralize trypsin. Gently shake the culture vessel to mix. | | 5. Transfer the cell suspension into a 15ml conical tube. Take a small aliquot of the suspension to count cells. Centrifuge the cell suspension 150 x g for 5 minutes at room temperature. | | 6. Carefully remove the supernatant. Add a volume of EPC freezing medium and re- suspend cells to 0.5 to 1 x 10e6 per ml. Dispense 1ml into each cryovial. | | 7. Place cryovials in a freezing container to slowly lower the temperature in a -80°C freezer. Transfer the vials to liquid nitrogen the next day for long term storage. | | Storage and Stability | Store at -20°C. Stable for 1 year from the date of receipt under proper storage condition. | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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