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You are here:Home » Culture Media » Media-Cell Culture » Endothelial Progenitor Outgrowth Cells, Human, Growth Medium Kit, BioAssay™

Endothelial Progenitor Outgrowth Cells, Human, Growth Medium Kit, BioAssay™

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Specifications

Catalog #E3010-68G
EPOC growth medium was designed and tested for human endothelium progenitor Outgrowth cells (EPOC). This medium includes two components EPOC basal medium (E3010-68G1) and EPOC growth medium supplement (E3010-68G2). The EPC growth medium supplement includes fetal bovine serum and human recombinant growth factors, and some lot-to- lot variability is unavoidable.
Each lot of this product is tested in cell culture to ensure its sterility. This product does not contain antibiotics.
Kit ComponentsE3010-68G1: Endothelial Progenitor Outgrowth Cells, Human, Basal Medium
E3010-68G2 Endothelial Progenitor Outgrowth Cells, Human, Growth Medium Supplement
Use of EPOC Growth Medium for Culturing EPOC1. Thaw the E3010-68G2 and add the entire volume (25ml) to the E3010-68G1 (500ml). Antibiotics may also be added if contamination is a concern.
2. Take the amount of EPOC growth medium needed and warm to 37°C in a water bath or incubator. Avoid frequent temperature change to the entire bottle of medium. The EPOC growth medium is stable for 1 month stored at 4°C.
3. Refer to manual for EPOC culture procedures using the EPOC growth medium. We recommend changing medium every 2 days for regular cell culture..
Storage and StabilityThe EPOC basal medium is stored at 4°C. A change in color or appearance of precipitate may indicate deterioration. The EPOC growth medium supplement is stored at -20°C. If taking aliquots from the original bottle is necessary, dispense the contents and re-freeze in small aliquots to avoid repeated freezing-thawing cycles. EPOC basal medium: Stable for 6 months from the date of receipt under proper storage condition. EPOC growth medium supplement: Stable for 6 months from the date of receipt under proper storage condition.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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