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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -eNOS (Nitric Oxide Synthase, Endothelial, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, Nitric Oxide Synthase 3, NOS Type III, NOSIII, NOS3)

Anti -eNOS (Nitric Oxide Synthase, Endothelial, Constitutive NOS, cNOS, EC-NOS,
Endothelial NOS, Nitric Oxide Synthase 3, NOS Type III, NOSIII, NOS3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IH
Catalog #E3010-84D
NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types neuronal NOS(nNOS) or brain NOS (bNOS); induciblr NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). This antibody reacts with eNOS.
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:70 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissues sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive ControlHUVEC cells, placenta
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the C-terminus of human eNOS.
SpecificityRecognizes human eNOS. Species Crossreactivity: mouse, rat, canine, porcine and bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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