Anti -eNOS (Nitric Oxide Synthase, Endothelial, Constitutive NOS, cNOS, EC-NOS,
Endothelial NOS, NOS Type III, NOSIII, Nos3)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| eNOS (Endothelial nitric-oxide synthase) is an important enzyme in the cardiovascular system. eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495. Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli. In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation. | | | Catalog # | E3010-85 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5-4ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Rat | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, 0.5% BSA, 0.01% thimerosal, 30% glycerol. | | Purity | Purified by affinity chromatography. | | Immunogen | Synthetic peptide corresponding to aa602 from rat eNOS. | | Specificity | Recognizes rat eNOS at ~140kD. Species Crossreactivity: human, mouse, porcine, equine, sheep, bovine and monkey. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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