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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -NOS III, phosphorylated (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial)

Anti -NOS III, phosphorylated (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3
Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial
NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #E3216
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot : 1:500-1:1000 detected phosphorylated eNOS in fetal bovine heart endothelial cell lysate.
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies I1904-39 IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined.
FormSupplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.1mM EDTA, and 0.05% sodium azide, before the addition of glycerol to 30%.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa106-118 of human eNOS (PRKLQGRPpSPGPP-C) with a C-terminal cysteine added for conjugation to KLH. The immunizing sequence shares 12/13 amino acids with porcine and bovine eNOS.
SpecificityRecognizes phosphorylated eNOS, Mr 132kD. Species Crossreactivity
porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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