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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -NOS III, NT (Endothelial Nitric Oxide Synthase, eNOS, NOS 3)

Anti -NOS III, NT (Endothelial Nitric Oxide Synthase, eNOS, NOS 3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IC
Catalog #E3218-01A
Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).
eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.
ApplicationsSuitable for use in Western Blot and immunocytochemical . Other applications not tested.
Recommended DilutionWestern Blot: 1:800
Immunocytochemistry: 1:400
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder. Reconstitute with 0.1ml PBS, 10mg/ml BSA.
PuritySerum
ImmunogenENOS (2-11 Cys12) covalently attached to a carrier protein.
SpecificityRecognizes human eNOS. Cross-reactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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