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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IH RIA

Catalog #	E3220-13
Applications	Suitable for use in Immunohistochemistry and RIA. Other applications not tested.
Recommended Dilution	Immunohistochemistry: 1:200-1:500; Recognizes all well-recognized enkephalin immunoreactive sites but does not bind to areas known to contain beta-endorphin or dynorphin.
	Optimal dilutions to be determined by the researcher.
Hybridoma	Secreted by a hybridoma formed by the fusion of a NSO/1 murine myeloma cell with a spleen cell from a BALB/c mouse immunized against Leu-enkephalin conjugated to bovine serum albumin.
Positive Control	Human small intestine
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	8.F.189
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a lyophilized powder with Thimersol. Reconstitute with 500ul sterile ddH2O.
Purity	Supernatant
Immunogen	Leu-enkephalin conjugated to BSA.
Specificity	Recognizes human Met5- and Leu5- Enkephalin. Displays a 40% crossreactivity with C-terminal-extended Met-enkephalin hexapeptides and 7% crossreactivity with the extended heptapeptide.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.