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You are here:Home » Antibodies » Abs to Neuron Specific Enolase » Anti -Enolase 2 (ENO2, Isoenzyme Neuron-specific Enolase)

Anti -Enolase 2 (ENO2, Isoenzyme Neuron-specific Enolase)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH
Enolase 2 (2-phospho-D-glycerate hydrolyase; also neural enolase and Y-enolase) is a 46kD member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Enolase 2 is 434aa in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently-linked homodimer, or heterodimer with a-enolase. Full-length human Enolase 2 is 99% aa identical to both mouse and canine Enolase 2. It shares 83% aa identity with human enolases # 1 and # 3.
Catalog #E3222-78A
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry, and Immunoprecipitation. Other applications not tested.
Recommended DilutionELISA: 0.5-1ug/ml Detection limit for Recombinant human Enolase 2 is ~1ng/well.
Immunoprecipitation: 25ug/ml
Western Blot (Non-reducing): 2ug/ml
Immunohistochemistry: 25ug/ml
Optimal dilutions to be determined by the researcher.
HybridomaMouse myeloma cells with B cells obtained from a mouse immunized with purified, E.coli-derived, recombinant human Enolase 2 (aa1-434).
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No9L824
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.0, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to aa1-434 of human Enolase 2 expressed in E. coli (P09140).
SpecificityRecognizes human Enolase 2. Species sequence homology: mouse, canine (99%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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