Ectonucleotide pyrophosphatase/phosphodiesterase 5 (ENPP-5) belongs to a group of nucleotide-metabolizing ecto-enzymes, which regulate the availability of extracellular nucleotides. It may also be involved in neuronal cell communication.
Applications
Suitable for use in Direct ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Immunoprecipitation: 25ug/ml will immunprecipitate recombinant human ENPP-5 from conditioned cell culture media Western Blot: 1ug/ml on recombinant human ENPP-5 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Pro25-Ser430 from human ENPP-5, expressed in NS0 cells. Species Sequence Homology: chimpanzee: 100%, canine: 88%, mouse and rat: 82%
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human ENPP-5. In Western Blot shows no cross-reactivity with recombinant human ENPP-2 or ENPP-7, or recombinant mouse ENPP-7.