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You are here:Home » Antibodies » Abs to Infectious Disease Enterovirus » Anti -Enterovirus

Anti -Enterovirus

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IC IF
Enterovirus primarily infects the alimentary tract and probably infects other organs through the blood vascular systems. This antibody recognizes most Enterovirus and poliovirus strains and some echovirus in tissue culture preparations.
Catalog #E3310-01B
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:10
Western Blot: 1:100-1:200
Immunocytochemistry: 1:20-1:40 for 30 min. at RT
Optimal dilutions to be determined by the researcher.
Positive Control
Immunofluorescence: Acetone-fixed HEp 2 cells infected with poliovirus type 1 (cytoplasmic staining pattern)
Western Blot: Sup T cells
Immunocytochemistry: Formalin-fixed, paraffin-embedded HEp 2 cells infected with poliovirus type 1 (cytoplasmic staining pattern)
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No5-D8/1
HostMouse
ConcentrationNot determined
FormSupplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
PuritySupernatant
ImmunogenCoxsackie virus B5.
SpecificityRecognizes Enterovirus VP1 peptide. Recognizes most Enterovirus and poliovirus strains and some echovirus in tissue culture preparations.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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