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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B IC IF

Enterovirus primarily infects the alimentary tract and probably infects other organs through the blood vascular systems. This antibody recognizes most Enterovirus and poliovirus strains and some echovirus in tissue culture preparations.
Catalog #	E3310-01B
Applications	Suitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution	Immunofluorescence: 1:10
	Western Blot: 1:100-1:200
	Immunocytochemistry: 1:20-1:40 for 30 min. at RT
	Optimal dilutions to be determined by the researcher.
Positive Control
	Immunofluorescence: Acetone-fixed HEp 2 cells infected with poliovirus type 1 (cytoplasmic staining pattern)
	Western Blot: Sup T cells
	Immunocytochemistry: Formalin-fixed, paraffin-embedded HEp 2 cells infected with poliovirus type 1 (cytoplasmic staining pattern)
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	5-D8/1
Host	Mouse
Concentration	Not determined
Form	Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
Purity	Supernatant
Immunogen	Coxsackie virus B5.
Specificity	Recognizes Enterovirus VP1 peptide. Recognizes most Enterovirus and poliovirus strains and some echovirus in tissue culture preparations.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.