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Eosin Methylene Blue Agar (Powder)


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Selective medium used to detect gram negative enteric bacteria. Contains both lactose and sucrose.
Catalog #E3325
DescriptionEosin Methylene Blue Agar was first developed by Holt-Harris and Teague. This formula contains both lactose and sucrose with the two indicator dyes, eosin Y and methylene blue. Escherichia and Enterobacter species can be easily distinguished from other Gram negative enteric bacilli using this medium. EMB Agar contains two mildly selective dyes, eosin Y and methylene blue, which interact under strong acidic conditions to impart metallic sheen to Escherichia coli colonies. In addition, E coli produces dark blue-black colonies. Other coliforms produce mucoid, pinkish brown colonies. Non-lactose fermenting bacteria Salmonella and Shigella, produce translucent or colorless colonies. Acinetobacter, Aeromonas, Pseudomonas, Plesiomonas and Yersinia also grow on this medium. Gram-positive bacteria are inhibited by eosin Y. The slow lactose fermenters also ferment sucrose which reduces the incidence of false-positive interpretations.
StorageStore the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original reddish-purple.
Light reddish-purple, homogeneous, free flowing powder.
Dark bluish to reddish-purple, clear to slightly turbid.
pH (25°C)
Microbiological Analysis
Enterococcus faecalis ATCC 29212Partial Inhibition, colorless
Escherichia coli ATCC 25922Growth, blue-black colonies with dark center and metallic sheen.
Salmonella typhimurium ATCC 14028Growth, colorless colonies
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesHH&T

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