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You are here:Home » Antibodies » Antibodies-Cell Markers » Anti -Eosinophil Major Basic Protein

Anti -Eosinophil Major Basic Protein


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Eosinophil major basic protein is felt to play an important role in eosinophil function and perhaps endothelial injury in hypereosinophilic states. Proteoglycan 2, bone marrow, also known as PRG2, is a human gene. The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases. A 117-residue protein that predominates in eosinophil granules. It is a potent enzyme against helminths and is toxic towards bacteria and mammalian cells in vitro. The eosinophil major basic protein also causes the release of histamine from mast cells and basophils, activates neutrophils and alveolar macrophages, and is directly implicated in epithelial cell damage, exfoliation and bronchospasm in asthma. Structurally the major basic protein (MBP) is similar to lectins (sugar-binding proteins), and has a fold similar to that seen in C-type lectins. However, unlike other C-type lectins (those that bind various carbohydrates in the presence of calcium), MBP does not bind either calcium or any of the other carbohydrates that this family recognize. Instead, MBP recognizes heparan sulfate proteoglycans. The structure of MBP is available from the Protein Data Bank, accession number 1h8u[2] and 2brs[3].
Catalog #E3330
ApplicationsSuitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: Fresh frozen (methanol:acetone)
Immunohistochemistry: FFPE: Treat with 0.1% trypsin before blocking.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No1.B.787
FormSupplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman eosinophil major basic protein (MBP)
SpecificityRecognizes human eosinophil major basic protein (14kD) irrespective of the stages of eosinophil activation and can be regarded as a “pan-eosinophil” marker. Recognizes the secreted pro-form of MBP (Pregnancy associated MBP, 23.8kD) and the mature form found in the matrix of the eosinophil large specific granule (13.8kD). Does not crossreact with equine, mouse or rat MBP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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