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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E IH IF

Eosinophil major basic protein is felt to play an important role in eosinophil function and perhaps endothelial injury in hypereosinophilic states. Proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein), also known as PRG2, is a human gene. The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases. A 117-residue protein that predominates in eosinophil granules. It is a potent enzyme against helminths and is toxic towards bacteria and mammalian cells in vitro. The eosinophil major basic protein also causes the release of histamine from mast cells and basophils, activates neutrophils and alveolar macrophages, and is directly implicated in epithelial cell damage, exfoliation and bronchospasm in asthma. Structurally the major basic protein (MBP) is similar to lectins (sugar-binding proteins), and has a fold similar to that seen in C-type lectins. However, unlike other C-type lectins (those that bind various carbohydrates in the presence of calcium), MBP does not bind either calcium or any of the other carbohydrates that this family recognize. Instead, MBP recognizes heparan sulfate proteoglycans. The structure of MBP is available from the Protein Data Bank, accession number 1h8u and 2brs.
Catalog #	E3330-01
Applications	Suitable for use in ELISA, Immunohistochemistry and Indirect Immunofluorescence. Other applications have not been tested.
Recommended Dilutions	ELISA: 1:800
	Indirect Immunofluorescence: 1:100-1:200
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	N/A
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	2Q2223
Host	Mouse
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid. No preservatives added.
Purity	Ascites
Specificity	Recognizes human eosinophil major basic protein from eosinophils among peripheral blood cells.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.