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You are here:Home » Antibodies » Abs to Tyrosine Kinases » Anti -Eck (EphA2, Mpk-5, Native Epithelial Cell Kinase, Eph receptor tyrosine kinase A2)

Anti -Eck (EphA2, Mpk-5, Native Epithelial Cell Kinase, Eph receptor tyrosine kinase A2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC
Catalog #E3360-01B
ApplicationsSuitable for use in Western Blot, Protein Kinase Assay, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml; Detected Eck in RIPA lysates from human A431 cells and previously from foreskin fibroblasts, murine 3T3/A31 and rat L6 cells. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with E3360-01B. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system
Protein Kinase Assay: Reported to have been used in an IP autophosphorylation assay, using a Mn-PIPES reaction buffer.
Immunoprecipitation: Reported to immunoprecipitate Eck from 500ug of a human breast epithelial cell line which had been lysed in TBS containing 1% Triton X-100. Use 1-4ug per reaction.
Immunocytochemistry: Reported to immunostain Eck in human, mouse and rat epithelial cells fixed with 3.7% formaldehyde solution and permeabilized with 0.5% Triton X-100 in TBS.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodyI1904-77B: IgG1 (HRP) Pab Gt x Mo
Positive Antigen ControlA0001-50: Non-stimulated A431 lysate
Control cellsCHO cell lysate, foreskin fibroblasts, 3T3/A31 cells, L6 cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.T.34
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenNative protein isolated by purification of phosphotyrosine-containing proteins
SpecificityRecognizes human Epithelial Cell Kinase (Eck) at 140kD.
Species CrossreactivityMouse, rat, bovine and canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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