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E3362-01M Rabbit Anti-EphA4, NT (Ephrin Type-A Receptor 4, Tyrosine-protein Kinase Receptor SEK, HEK8, EPH-like Kinase 8, EK8, Tyrosine-protein Kinase TYRO1, HEK8, SEK, TYRO1)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P54764
Isotype
IgG
Grade
Purified
Applications
E FC IF IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Applications
Suitable for use in ELISA, Western Blot, Indirect Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Immunofluorescence: 1:100 Flow Cytometry (Indirect): 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 40-70 in the N-terminal region of human EphA4. Species Sequence Homology: Xenopus, chicken and mouse
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human EphA4
References
1. Prevost, N., et al., Proc. Natl. Acad. Sci. USA 99(14):9219-9224 (2002). 2. Xu, Q., et al., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 355(1399):993-1002 (2000). 3.Holder, N., et al., Development 126(10):2033-2044 (1999). 4. Zhou, R., Pharmacol. Ther. 77(3):151-181 (1998). 5. Fox, G.M., et al., Oncogene 10(5):897-905 (1995).
USBio References
No references available
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