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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -EphB4 Receptor Tyrosine Kinase

Anti -EphB4 Receptor Tyrosine Kinase

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH
The Eph receptors are the largest family of receptor tyrosine kinases. They can be grouped into two subclasses: the EphA and the EphB receptors. The ligands for the Eph receptors are the ephrins. The EphA receptors bind ephrin-A ligands, which are tethered to the membrane by a GPI linkage, and the EphB receptors bind ephrin-B ligands, which have a membrane spanning region. The Eph receptors are widely expressed during embryonic development. Ephrins and Eph receptors have been implicated in many developmental processes including neuronal network formation, guidance of cell migration, and axonal pathfinding. They remain expressed at lower levels in adult tissues and are upregulated in pathological conditions such as cancer. EphB4 is expressed in epithelial cells and in endothelial cells of embryonic veins, where its signaling mediates cell migration and proliferation via the PI3K pathway. It is upregulated in several types of tumors, including mammary tumors, colon cancer, and endometrial cancer. EphB4 has previously been designated Htk, Myk1, Tyro11 and Mdk2.
Catalog #E3373-05D
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1-3ug/ml
Immunoprecipitation: 5ug/IP reaction
Immunohistochemistry: 10ug/ml. Formalin-fixed, paraffin-embedded tissues require heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0 prior to staining.
Optimal dilutions to be determined by the researcher.
Positive ControlsWestern Blot: EphB4-transfected 293T cells and human MDA-MB-231 breast carcinoma cell lysates
Immunohistochemistry: Human colon cancer tissues
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6A76
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein derived from the C-terminal region of the human EphB4 protein.
SpecificityRecognizes endogenous human EphB4 receptor at ~110-120kD, with weak crossreactivity with the EphB2 receptor.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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