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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -EphB4 Receptor Tyrosine Kinase (Eph Receptor B4, EphB4, Hepatoma Transmembrane Kinase, HTK, MYK1, TYRO11)

Anti -EphB4 Receptor Tyrosine Kinase (Eph Receptor B4, EphB4, Hepatoma Transmembrane
Kinase, HTK, MYK1, TYRO11)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B
EphB4 is a member of the Eph receptor family, which bind the ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains juxtamembrane motif with two tyrosines, which are the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxyl terminus, which includes one conserved tyrosine. Ligand recognition and binding leads to activation of intrinsic kinase activity. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands can bind the receptor, but do not induce receptor autophosphorylation and activation. The Eph receptors and ephrin ligands display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands. Ephs and ephrins play a significant role in angiogenesis.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Catalog #E3373-05F
Recommended DilutionWestern Blot: 1:100-1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9G66
HostRat
SourceMouse
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant mouse EphB4 extracellular domain
SpecificityRecognizes mouse EphB4. In this format, this antibody does not crossreact with recombinant mouse EphA2, EphA3, EphA4, EphA6, EphA7 or EphB6.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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