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Specifications

Catalog #	E3374-07
Biological Activity	The optimal EGF concentration in cell culture is 0.1–25ng/ml depending on cell type.
Biological Activity	5ng/ml of this lot supports a 2.5- fold increase in cell number in a 5-day assay using human foreskin broblasts. Previously, 5ng/ml EGF supported a 2.2-fold increase in cell number in a 5-day assay using normal rat kidney broblasts (NRK-49F).
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight	16kDa
Source	Mouse
Purity	Single band on SDS-PAGE gel at 16kDa (1ug run under non-reducing conditions and Coomassie blue stained).
Form	100ug/vial of lyophilized powder. Lyophilized from 157ul of 20mM ammonium acetate. Quantitated by spectrophotometry using the extinction coef cient for EGF [E (1%, 280nm)]= 30.9
Important Note	This product is intended for research use only, not for use in human, therapeutic or diagnostic applications.