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Specifications

Catalog #	E3374-08
Biological Activity	The optimal EGF concentration in cell culture is 0.1-25ng/ml depending on cell type. 5ng/ml of EGF supports a 2.4-fold increase in cell growth in a 5-day assay using human foreskin broblasts (HFF).
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or in culture medium. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight	16kD
Source	Male mouse submaxillary glands.
Purity	~99% by SDS polyacrylamide gel electrophoresis.
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from 0.01M sodium acetate. Reconstitute with sterile ddH2O or in culture medium.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.