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You are here:Home » Molecular Biology » Growth Factors-EGF » Epidermal Growth Factor, Murine (EGF)

Epidermal Growth Factor, Murine (EGF)

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Specifications

Catalog #E3374-08
Biological ActivityThe optimal EGF concentration in cell culture is 0.1-25ng/ml depending on cell type. 5ng/ml of EGF supports a 2.4-fold increase in cell growth in a 5-day assay using human foreskin broblasts (HFF).
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or in culture medium. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight16kD
SourceMale mouse submaxillary glands.
Purity~99% by SDS polyacrylamide gel electrophoresis.
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from 0.01M sodium acetate. Reconstitute with sterile ddH2O or in culture medium.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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