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Epidermal Growth Factor, Recombinant, Mouse (EGF, beta-Urogastrone, URG)
Epidermal Growth Factor, Recombinant, Mouse (EGF, beta-Urogastrone, URG)
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| Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant murine EGF is a 6.0kD protein containing 53 amino acid residues. | | | Catalog # | E3374-11 | | Amino Acid Sequence | NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR | | Biological Activity | The ED50 as determined by a cell proliferation assay using BALB/c 3T3 cells is 0.1ng/ml, corresponding to a specific activity of 1x10e7 units/mg. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 6kD | | Source | E. coli. | | Purity | 8% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug). | | Form | Supplied as a lyophilized powder from 10mM sodium phosphate, pH 8.2. Reconstitute sterile ddH2O to 0.5-1mg/ml, which can then be further diluted to other aqueous solutions | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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