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You are here:Home » Growth Factors, Cytokines » Growth Factors-EGF » Epidermal Growth Factor, Recombinant, Rat (EGF)

Epidermal Growth Factor, Recombinant, Rat (EGF)

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Specifications

EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF- and VGF (vaccinia virus growth factor). Recombinant rat EGF is a 6.2kD globular protein containing 54 amino acid residues including 3 intramolecular disulfide-bonds.
Catalog #E3374-11C
Biological ActivityThe ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is < 0.1ng/ml, corresponding to a specific activity of >1x10e7 units/mg.
Amino Acid SequenceMNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, carrier protein. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of OriginUSA
SourceE. coli
Purity98% by SDS-PAGE
FormSupplied as a lyophilized powder. Reconstitute with sterile ddH2O, carrier protein to a final concentration of 0.1-1mg/ml. Do not vortex.
Endotoxin<0.1ng/ml (1EU/ug)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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