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You are here:Home » Antibodies » Abs to Receptors » Anti -Epidermal Growth Factor Receptor (erbB, EGFR), Extracellular Domain

Anti -Epidermal Growth Factor Receptor (erbB, EGFR), Extracellular Domain

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP FC
EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3, -4 or HER-1, -2, -3, -4. It binds to epidermal growth factor (EGF), transforming growth factor-a
(TGFa), heparin binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head and neck, esophagus, cervix, vulva, ovary and endometrium. It is predominantly present in squamous cell carcinomas.
Catalog #E3375-03
ApplicationsSuitable for use in Flow Cytometry, Immunoprecipitation, Affinity Purification, Blocking of EGF/TGFa-induced Activation of EGFR, Inhibition of Cell Proliferation (A431 cells), Arrests tumor growth in vivo. Not suitable for Western Blot. Other applications have not been tested.
Recommended DilutionImmunoprecipitation: Native only.
Affinity Purification: Recommended to use E3375-03X
Blocking of EGF/TGFa-induced Activation of EGFR: 0.5ug/ml, cells induced by 30ng/ml EGF. Recommended to use E3375-03X.
Arrests tumor growth in vivo: Recommended to use E3375-03X.
Optimal dilutions to be determined by the researcher.
Positive ControlA431 cells
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No0.N.265
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 15mM sodium azide, before the addition of glycerol to 40%. Also available without BSA and azide. See E3375-03X.
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified EGFR from A431 cells. Cellular Localization: Cell membrane.
SpecificityRecognizes the 170kD (intact) and 145kD (VIII variant) forms of EGF receptor. The epitope maps to the extracellular domain of EGFR. Blocks EGF/TGFa-induced activation of EGFR (1,2). No effect on tyrosine kinase activity of the receptor. Inhibits the binding of antibody E3375-04 (-04X). Does not crossreact with erbB-2, erbB-3, or erbB-4. Species crossreactivity: Human and canine. Does not cross react with mouse or rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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