Anti -Epidermal Growth Factor Receptor (erbB, EGFR), Extracellular Domain
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IH FC IF |
|
| EGFR is a type I recpetor tyrosine kinase with sequence homology to erb-B 1, 2, 3, 4, or HER 1, 2, 3, 4. It binds to epidermal growth factor, transforming growth factor-a, heparin binding EGF, amphiregulin, betacellulin, and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head and neck, esophagus, cervix, vulva, ovary and endometrium. It is predominantly present in squamous cell carcinomas. | | | Catalog # | E3375-07 | | Applications | Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. | | Recommended Dilution | Western Blot: 0.5-1ug/ml for 2 hours at RT | | Immunoprecipitation (Native only): 2ug/mg protein lysate using Protein G. | | Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires digestion with protease XXV at 1mg/ml PBS for 5 minutes at 37°C. | | Optimal dilutions to be determined by the researcher. | | Positive Control | A431 cells. Placenta or squamous cell carcinoma. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 0.N.269 | | Host | Mouse | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide, before the addtion of glycerol to 40%. Also available without BSA and azide. See E3375-07X. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | HC2 20 d2 cells. Cellular Localization: Cell membrane. | | Specificity | Recognizes the 170kD (wild type) and 145kD (VIII variant) forms of human EGF receptor. The epitope maps to the extracellular domain of EGFR. Does not react with mouse or rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
