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You are here:Home » Antibodies » Abs to Receptors » Anti -Epidermal Growth Factor Receptor (erbB, EGFR), Extracellular Domain (BSA & Azide Free)

Anti -Epidermal Growth Factor Receptor (erbB, EGFR), Extracellular Domain (BSA & Azide
Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH FC IF
EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3, -4 or HER-1, -2, -3, -4. It binds to epidermal growth factor (EGF), transforming growth factor-a (TGF-a), heparin binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is over expressed in tumors of breast, brain, bladder, lung, gastric, head and neck, esophagus, cervix, vulva, ovary and endometrium. It is predominantly present in squamous cell carcinomas.
Catalog #E3375-07X
ApplicationsSuitable for use in ELISA, Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 0.5-1ug/ml for 2 hrs at RT. Suitable for Western blot of extracellular domain of EGFR.
Immunoprecipitation (Native only): 2ug/mg protein lysate. Use Protein G.
Immunohistochemistry (Formalin/ Paraffin): 2-4ug/ml for 30 mniutes at RT. Staining of formalin-fixed tissues requires digestion with Protease XXV at 1mg/ml PBS for 5 min at 37°C. (Note: E3375-06B/ E3375-06BX are more suitable for use in IHC).
Optimal dilutions to be determined by the researcher.
Positive ControlA431 cells, placenta or squamous cell carcinoma.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.N.269
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No preservatives and no stabilizing proteins added. Also available with BSA and azide. See Cat # E3375-07.
PurityPurified by Protein G affinity chromatography.
ImmunogenHC2 20 d2 cells. Cellular Localization
SpecificityRecognizes the 170kD (wild type) and 145kD (VIII variant) forms of human EGF receptor. Species Crossreactivity: Does not crossreact with mouse or rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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