| EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2,-3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head and neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas. |
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| Catalog # | E3375-10F |
| Applications | Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Not suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilutions | Flow Cytometry: 1:50-1:100. Use 10ul to label 10e6 cells in 100ul. |
| Immunohistochemistry: Frozen and paraffin sections |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Reagent | I1904-49H: IgG, F(ab')2, H&L X-Adsorbed (HRP) RbxRt |
| I1904-47H: IgG, H&L (HRP) Pab GtxRt |
| Positive Control | Breast carcinoma |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 3H2090 |
| Host | Rat |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. |
| Purity | Purified by Ion Exchange chromatography. |
| Immunogen | Extracellular domain of human EGF-receptor from head and neck carcinoma. |
| Specificity | Recognizes the human epidermal growth factor receptor (EGF-R), which is overexpressed in a high proportion of breast cancer cells and in a range of other carcinomas. High level expression of EGFR is often associated with advanced disease and poor prognosis. Binds to epitope B from EGFR and has an affinity of 6.7x10e-9M. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
