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You are here:Home » Antibodies » Abs to Receptors » Anti -Epidermal Growth Factor Receptor (erbB, EGFR) (FITC)

Anti -Epidermal Growth Factor Receptor (erbB, EGFR) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Highly Purified FC
The EGF receptor (EGFR), a 180kD transmembrane tyrosine kinase, is part of the ErbB family which mediate ligand induced signaling as heterodimers. Ligand binding results in activation of the intrinsic kinase activity and autophosphorylation on tyrosine residues within and surrounding the kinase domain. The phospho-tyrosine residues serve as docking sites for adapter proteins that nucleate signaling complexes that result in the activation of the Ras, PI-3 kinase and PLC-g pathways. EGFR, which transduces the biological effects of EGF and TGF-a, is frequently over-expressed in solid tumors.
Catalog #E3375-10F1
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:10, Use 10ul to label 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
Recommended Negative ControlsI1904-82E2: IgG2a Rat Negative Control (FITC)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H2090
HostRat
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified by Ion Exchange chromatography.
ImmunogenExtracellular domain of human EGF-receptor from head and neck carcinoma.
SpecificityRecognizes the human epidermal growth factor receptor (EGF-R), which is overexpressed in a high proportion of breast cancer cells and in a range of other carcinomas. Binds to epitope B from EGFR (1, 2) and has an affinity of 6.7 x 10e-9 M.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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