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You are here:Home » Antibodies » Abs to Receptors » Anti -Epidermal Growth Factor Receptor (erbB, EGFR)

Anti -Epidermal Growth Factor Receptor (erbB, EGFR)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC
Catalog #E3375-11
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested.
Recommended DilutionImmunocytochemistry: 5ug/ml. Demonstrates positive immunostaining for EGF receptor in A431cells fixed with 4% paraformaldehyde followed by 100% methanol.
Western Blot: 0.5-2ug/ml. Detects EGF receptor (~180kD) in RIPA lysates from human A431 cells under reducing conditions. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with E3375-11 (1ug/ml). Proteins were visualized using a goat anti-mouse IgG labeled with HRP and a chemiluminescence detection system.
Immunoprecipitation: 4ug. Immunoprecipitates EGF receptor from 500ug of A431 RIPA lysate.
Optimal dilutions to be determined by the researcher.
Positive Antigen ControlA0001-50: A431 Cell Lysate, Non-Stimulated
HybridomaX63-Ag8.653x Balb/c-spleen cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No0.T.36
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenA431 membrane proteins.
SpecificityRecognizes the sequence of Ala351-Asp364 of human EGF receptor, Mr 180kD. Species crossreactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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