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You are here:Home » Antibodies » Abs to Receptors » Anti -Epidermal Growth Factor Receptor (erbB, EGFR), Neutralizing

Anti -Epidermal Growth Factor Receptor (erbB, EGFR), Neutralizing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IC N
Catalog #E3375-14
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Neutralization. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: Acetone-fixed tissues.
Neutralization of Biological Activity: 10ug/ml causes a 74% reduction in cell number in a 5 day cell proliferation assay using A431 cells.
Western Blot: 0.5-2ug/ml detects non-reduced EGF receptor in RIPA lysates from non-stimulated human A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with E3375-14 (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemilum-inescence detection system.
Immunoprecipitation: 4ug immunoprecipitates the EGF receptor from 500ug of non-stimulated A431 RIPA lysate.
Immunocytochemistry: 10ug/ml shows positive immunostaining for the EGF receptor in A431 cells fixed with 4% para-formaldehyde.
Optimal dilutions to be determined by the researcher.
Biological ActivityInhibits A431 cell growth. Recognizes an antigenic determinant located on the extracellular domain of the receptor.
Positive Antigen ControlA0001-50: A431 Cells, Non-Stimulated Use 20ug per lane for minigels.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O.. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.T.37
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder from 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride. Reconstitute with 250ul sterile ddH2O. Further dilute with PBS, 5mg/ml BSA.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman A431 membrane proteins.
SpecificityRecognizes the external domain of EGF receptor on all human cells at ~180kD. Competes with EGF and TGF-a for binding on human cells. Recognizes both phosphorylated and non-phosphorylated human EGF receptors. Species crossreactivity: does not react with EGF receptors on mouse or rat cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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