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You are here:Home » Antibodies » Abs to Receptors » Anti -Epidermal Growth Factor Receptor (erbB, EGFR) (nonPhospho-Y1173)

Anti -Epidermal Growth Factor Receptor (erbB, EGFR) (nonPhospho-Y1173)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
Catalog #E3375-16
ApplicationsSuitable for use in Western Blot, Immunoprecipitation.
Recommended DilutionWestern Blot: 0.5-2ug/ml detects the EGF receptor (unphosphorylated at Y1173) in RIPA lysates from unstimulated HFF cells. Lysates from EGF stimulated and unstimulated HFF cells, 10ug per lane, were resolved by electrophoresis and transferred to nitro-cellulose. Lanes 1 and 2 were probed with anti-EGF receptor and visualized using a rabbit anti-sheep secondary antibody conjugated to HRP. Lanes 3 and 4 were probed with anti-EGF-R (non-phospho-Y1173) (1ug/ml) and visualized using a goat anti-mouse secondary antibody conjugated to HRP. A chemiluminescence detection system was used. (EGF-R 175 kD).
Immunoprecipitation 1-10ug of this antibody is reported to immunoprecipitate the EGF receptor (unphosphorylated at Y1173) from 10e6 vanadate treated A431 cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.T.197
HostMouse
SourceHuman
Concentration1mg/ml
FormSupplied as a liquid in 0.07M Tris-glycine, pH 7.4, 0.105M sodium chloride, 0.05% sodium azide, 30% glycerol. Also available without azide and glycerol. See E3375-16X.
PurityPurified by subsequent thiophilic adsorption and size exclusion chromatography
ImmunogenKLH-conjugated, synthetic peptide containing the unphosphorylated Tyr1173 motif (… NAE[Y]LR… ) of the human EGF receptor.
SpecificityRecognizes the motif NAEYLR of the EGF-R (unphosphorylated at Tyr1173). Does not recognize the phosphorylated (pTyr1173) form of the EGF receptor. Reacts with human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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