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You are here:Home » Culture Media » Media-Cell Culture, Serum Free » Epithelial (Human Breast) Serum Free Media (BRFF BMZERO)

Epithelial (Human Breast) Serum Free Media (BRFF BMZERO)


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BRFF-BMZERO™ is a complete serum-free medium designed to grow human breast cells. This SFM was originally used for establishing new epithelial cell lines from explants of human breast tissue and to grow immortalized normal breast cell lines. This medium is a good first choice when switching from serum containing medium to serum free or for the cultivation of primary cultures. For optimum cell attachment, use FNC Coating Mix®.
Catalog #E3400-05
Ready-to-use with all necessary growth factors. Antibiotic-free.
Storage and StabilityFor long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
CAS NumberN/A
FormSupplied as a liquid.
pH7.2-7.5 (5% CO2 atmosphere is recommended for culture).
Osmolality270-300mOsM Unit
Functional TestGrowth of cell line 267-B1 to confluence. One vial containing 1-5x10e6 celss is quick thawed, washed with 10ml of medium, resuspended in 10ml of medium and applied to a T-25 flask pre-coated with FNC Coating Mix®. The culture is incubated at 37°C in a 5% CO2 incubator.
Sterility TestPass
SpecificationDetection of microbial growth
Test Sample0.1ml into each of the following media (in triplicate); Nutrient Broth,
Thioglycollate Medium.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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