Anti -Epithelial Cell Surface Antigen (ESA, Adenocarcinoma-associated Antigen, CD326,
CD326 Antigen, Cell Surface Glycoprotein Trop1, Cell Surface Glycoprotein Trop-1, CO-17A,
CO17-1A, Epithelial Cellular Adhesion Molecule, Ep CAM, EpCAM, Ep-CAM, Epithelial Gly
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IH FC |
|
| The epithelial cell adhesion molecule (Ep-CAM) is a 38kD cell surface antigen otherwise known as CD326, MOC31 and Ber-EP4. CD326 is a type 1 transmembrane glycoprotein. It is expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells. | | | Catalog # | E3405-50 | | Applications | Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. | | Recommended Dilutions | Immunohistochemistry (Frozen and paraffin):1:200 for paraffin sections. Requires protein digestion pretreatment (e.g. trypsin, pronase) of paraffin sections. | | Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul | | Western Blot: Detects a band at ~38kD in HT-29 cell lysates. | | Optimal dilutions to be determined by the researcher. | | Positive Control (Immunohistochemistry) | Large bowel | | Recommended Negative Control | I1904-78R: IgG1 Murine Negative Control | | Hybridoma | Sp2/0 myeloma cells with spleen cells from Balb/c mice. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 10B1416 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, 0.09% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | H69 cell line (small cell lung carcinoma). | | Specificity | Recognizes the human epithelial cell adhesion molecule (Ep-CAM). at ~38kD. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
|
|
Questions or comments about this product? Inquire here. |
|
|
Download Specifications for Anti -Epithelial Cell Surface Antigen (ESA, Adenocarcinoma-associated Antigen, CD326, CD326 Antigen, Cell Surface Glycoprotein Trop1, Cell Surface Glycoprotein Trop-1, CO-17A, CO17-1A, Epithelial Cellular Adhesion Molecule, Ep CAM, EpCAM, Ep-CAM, Epithelial Gly |
External Links
|
