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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Epithelial Cell Surface Antigen (ESA, Adenocarcinoma-associated Antigen, CD326, CD326 Antigen, Cell Surface Glycoprotein Trop1, Cell Surface Glycoprotein Trop-1, CO-17A, CO17-1A, Epithelial Cellular Adhesion Molecule, Ep CAM, EpCAM, Ep-CAM, Epithelial Gly

Anti -Epithelial Cell Surface Antigen (ESA, Adenocarcinoma-associated Antigen, CD326,
CD326 Antigen, Cell Surface Glycoprotein Trop1, Cell Surface Glycoprotein Trop-1, CO-17A,
CO17-1A, Epithelial Cellular Adhesion Molecule, Ep CAM, EpCAM, Ep-CAM, Epithelial Gly

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC
The epithelial cell adhesion molecule (Ep-CAM) is a 38kD cell surface antigen otherwise known as CD326, MOC31 and Ber-EP4. CD326 is a type 1 transmembrane glycoprotein. It is expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells.
Catalog #E3405-50
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Frozen and paraffin):1:200 for paraffin sections. Requires protein digestion pretreatment (e.g. trypsin, pronase) of paraffin sections.
Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul
Western Blot: Detects a band at ~38kD in HT-29 cell lysates.
Optimal dilutions to be determined by the researcher.
Positive Control (Immunohistochemistry)Large bowel
Recommended Negative ControlI1904-78R: IgG1 Murine Negative Control
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No10B1416
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenH69 cell line (small cell lung carcinoma).
SpecificityRecognizes the human epithelial cell adhesion molecule (Ep-CAM). at ~38kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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