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You are here:Home » Molecular Biology » Growth Factors-EGF » Epidermal Growth Factor, Recombinant, Human (EGF, beta-Urogastrone, URG)

Epidermal Growth Factor, Recombinant, Human (EGF, beta-Urogastrone, URG)

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Specifications

Human EGF is produced by genetically engineered yeast, and purified by sequential chromatography and reverse-phase HPLC. This polypeptide is identical to gamma-urogastrone, or equivalent to human.
Catalog #E3412
SourceEGF1-52
Biological ActivityThe mitogenic activity of human EGF is measured by the stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells. In addition, EGF activity has been determined by a receptor-binding assay (RBA) using A-431 cells.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to with sterile dH2O or buffer, aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceEGF1-52
PurityPurified. 97% pure by N-terminal aa sequencing, aa composition, HPLC analysis, and SDS gel electrophoresis
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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