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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1, 17-1A antigen, EPG34)

Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40,
Ep-CAM, MK-1, 17-1A antigen, EPG34)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Ep-CAM (also described as 17-1A antigen or EPG34). This antigen is widely expressed on cells of epithelial origin and tumors derived therefrom. This clone represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative. Polypeptide reacting Mr 40,000 human epithelium-specific cell surface glycoprotein (Ep-CAM). Reactivities on Cultured All carcinoma cell lines tested so far; particularly strong reaction.
Catalog #E3414-04
ApplicationsSuitable for use in Immunoblot, Immunofluorescence microscopy, Immunohistochemistry. Suitable for frozen and paraffin-embedded tissue, cell sorting, cytological material. Pretreatment With paraffin embedded sections, protease pretreatment is required prior to antibody application
Recommended DilutionImmunohistochemistry: 1:10 with PBS, pH 7.4; Incubation Time 1 h at RT; extended with paraffin.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F209
HostMouse
SourceHuman
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder. Reconstitute to 1ml final volume.
PurityPurified by Protein A affinity chromatography.
ImmunogenHT-29 colon carcinoma cell line.
SpecificityAntigen Recognized in Human Species (tested so far). Cell Lines (tested so far) with colon carcinoma cell lines (HT-29, WiDr, SW1116). Recognizes the epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen or EPG34).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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