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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1, 17-1A antigen, EPG34) (FITC)

Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40,
Ep-CAM, MK-1, 17-1A antigen, EPG34) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IC IF
Ep-CAM (also described as 17-1A antigen or EPG34) is widely expressed on cells of epithelial origin and tumors derived therefrom. This clone represents an excellent marker to discriminate epithelial from mesothelial structures.
Catalog #E3414-04A
ApplicationsSuitable for use in Immunofluorescence Microscopy, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Frozen/paraffin): 1:10 with PBS, pH 7.4. Protease pretreatment of paraffin embedded sections is required. Incubate for 1 hr at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F209
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid, 0.09% sodium azide. Labeled with FITC.
PurityPurified by Protein A affinity chromatography.
ImmunogenHT-29 colon carcinoma cell line.
SpecificityRecognizes the human Epithelial Specific Antigen. Reacts with carcinoma cells lines. Strong reaction seen in colon carcinoma cell lines (HT-29, WiDr, SW1116). Does not react with a subset of squamous cell carcinoma.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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