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Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1)
Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Supernatant |
B IP IH |
|
| Catalog # | E3414-05B | | Recommended Secondary Reagents | F(ab')2 rabbit anti-mouse IgG HRP | | Fusion Partners | Spleen cells from immunized mice were fused with cells of the mouse SP2/0 myeloma cell line. | | Recommended Positive Control Tissue | Large Bowel | | Recommended Negative Controls | Mouse IgG1 Negative Control | | Applications | Immunohistochemistry: (frozen)- 1:50-1:100, (paraffin)- 1:50 -1:100 | | Western Blotting: 1:25-1:50 | | Immunoprecipitation | | Optimal working dilutions to be determined by researcher. | | Immunohistochemistry | Requires protein digestion pre-treatment of paraffin sections e.g. trypsin. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 3H2097 | | Host | Mouse | | Source | Human | | Concentration | As reported | | Form | Tissue Culture Supernatant lyophilized with 0.1% sodium azide. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O. | | Purity | Tissue culture supernatant. | | Immunogen | HG9 cell line (small cell lung carcinoma). | | Specificity | Recognizes human epithelial specific antigen (ESA), a 40kD cell surface glycoprotein expressed by the majority of epithelial cells. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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